NSCLC Spheroid Formation and Analysis

The process is referred to the previous study [18 (link)]. Briefly, NSCLC cells were maintained in DMEM-F12 medium containing B27 (Sigma, 20 ng/ml) and EGF (BD Biosciences, San Jose, CA, 10 ng/ml) in non-adherent 24-well plates (Corning, NY) at 1000 cells/well for 10 days. After then, spheroids with more than 50 μm were photographed and counted. For analysis on spheroids, spheroids were collected, trypsinized and re-seeded in plates. The spheroid-derived cells were incubated until the end of each experiment, and fresh spheroids were collected for each experiments.

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Jin L., Wu Z., Wang Y, & Zhao X. (2020). Cryptotanshinone attenuates the stemness of non-small cell lung cancer cells via promoting TAZ translocation from nuclear to cytoplasm. Chinese Medicine, 15, 66.

Publication 2020

EGF non-adherent 24-well plates

Cells Nsclc

Corresponding Organization :

Other organizations : Nanjing Medical University, Jiangsu Province Hospital

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Variable analysis

independent variables

Cell culture conditions (DMEM-F12 medium containing B27 and EGF)

dependent variables

Spheroid formation (number of spheroids with more than 50 μm diameter)

control variables

Cell density (1000 cells/well)
Culture duration (10 days)

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