The human ATLL cell lines, ATL-T and ED, were previously established by Taylor and
Matsuoka.22 (link) The T-cell leukemia cell
line MOLT-4 was purchased from the RIKEN Cell Bank (WAKO, Japan). Control TF-1 cells
(TF-1CT) and TF-1 cells expressing M-CSFR
(TF-1MCSFR) were previously established by Suzu.23 (link) Cells were maintained in RPMI1640
supplemented with 10% fetal bovine serum. Mycoplasma testing was performed using a
polymerase chain reaction (PCR) detection kit (Takara Bio Inc., Otsu, Japan). Conditioned
medium (CM) from cell lines was obtained as described previously.24 (link) Recombinant M-CSF was a gift from Morinaga Milk Industry
(Kanagawa, Japan). IL-34 was purchased from R&D Systems (Minneapolis, MN, USA).
PLX3397 (pexidartinib) was obtained from APExBio (Houston, TX, USA). Cell block samples of
cell lines were prepared as described previously.25 (link)
Matsuoka.22 (link) The T-cell leukemia cell
line MOLT-4 was purchased from the RIKEN Cell Bank (WAKO, Japan). Control TF-1 cells
(TF-1CT) and TF-1 cells expressing M-CSFR
(TF-1MCSFR) were previously established by Suzu.23 (link) Cells were maintained in RPMI1640
supplemented with 10% fetal bovine serum. Mycoplasma testing was performed using a
polymerase chain reaction (PCR) detection kit (Takara Bio Inc., Otsu, Japan). Conditioned
medium (CM) from cell lines was obtained as described previously.24 (link) Recombinant M-CSF was a gift from Morinaga Milk Industry
(Kanagawa, Japan). IL-34 was purchased from R&D Systems (Minneapolis, MN, USA).
PLX3397 (pexidartinib) was obtained from APExBio (Houston, TX, USA). Cell block samples of
cell lines were prepared as described previously.25 (link)
