Protocol detail

Cell Lines and Antibody Sources

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HeLa (H1, #CRL-1958), HT-29 (#HTB-38), CHO-C6, and 293T (#CRL-3216) cells were originally obtained from ATCC. They tested negative for mycoplasma contamination, but have not been authenticated. Huh7 and Huh7 LDLR^−/− cells were kindly provided by Y. Matsuura^{36 (link)}. The following mouse monoclonal antibodies were purchased from the indicated vendors: RAC1 (23A8, Abcam), non-glucosylated RAC1 (Clone 102, BD Biosciences), β-actin (AC-15, Sigma), and HS (F58–10E4, mouse IgM, Amsbio). Rabbit monoclonal IgG against LDLR (EP1553Y) was purchased from Abcam. Chicken polyclonal IgY (#753A) against TcdA was purchased from List Biological Labs. Statistical analysis was performed using OriginPro 8 (V8.0724, OriginLab Corp.) software.

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Tao L., Tian S., Zhang J., Liu Z., Robinson-McCarthy L., Miyashita S.I., Breault D.T., Gerhard R., Oottamasathien S., Whelan S, & Dong M. (2019). Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nature microbiology, 4(10), 1760-1769.

Publication 2019

HeLa (H1, HT-29 CHO-C6 RAC1 (23A8, non-glucosylated RAC1 (Clone 102, β-actin (AC-15, Rabbit monoclonal IgG against LDLR (EP1553Y) OriginPro 8

Actin Biological Cells Chicken Clone Hela Ht 29 Ldlr Monoclonal antibodies Mouse Mycoplasma Rabbit

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Variable analysis

independent variables

Cell lines: HeLa (H1, #CRL-1958), HT-29 (#HTB-38), CHO-C6, 293T (#CRL-3216), Huh7, and Huh7 LDLR-/- cells

dependent variables

Mycoplasma contamination status
Authentication status

control variables

Mycoplasma contamination testing
Cell line source (ATCC, Y. Matsuura)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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