Western Blotting of Key Signaling Proteins

Western blotting was carried out as described previously [37 (link)]. The following primary antibodies were used for western blotting: anti-MED23 (BD, 550492), anti-MED16 (Bethyl, A303-668A, RRID: AB_11205632), anti-MED24 (Bethyl, A301-472A, RRID: AB_999675), anti-phosphorylated-Erk1/2 (Thr202/Tyr204) (Santa Cruz, sc-16982), anti-Erk (K-23) (Santa Cruz Biotechnology, sc-94-G, RRID: AB_631456), anti-phosphorylated-Akt (S473) (Cell signaling, 9271), anti-Akt (Cell signaling Technology, 9272, RRID: AB_329827), anti-Ets1 (Santa Cruz, sc-350x), anti-Sprouty2 (Santa Cruz, sc-30049), anti-Src (Cell signaling Technology, 2108s, RRID: AB_331137) and β-Actin (Sigma, A5441).

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Fu X., Liu S., Cao D., Li C., Ji H, & Wang G. (2024). Med23 deficiency reprograms the tumor microenvironment to promote lung tumorigenesis. British Journal of Cancer, 130(5), 716-727.

Publication 2024

anti-phosphorylated-Akt (S473) anti-Akt anti-Ets1 anti-Sprouty2 anti-Src β-Actin

Corresponding Organization :

Other organizations : Zhongshan Hospital, Fudan University, Shanghai Jiao Tong University, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of MED23, MED16, MED24, phosphorylated-Erk1/2, Erk, phosphorylated-Akt, Akt, Ets1, Sprouty2, and Src proteins

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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