Isolation of Proarteriogenic Monocytes

We used TIE2 as a marker of proarteriogenic Mo/MΦs, and cells were isolated according to previously published methodology (Supplemental Figure 4) (15 (link)). Briefly, peripheral blood mononuclear cells were isolated from 100 mL of venous blood by density centrifugation (at 400g, spun for 30 minutes at 4°C) using Ficoll-Paque (GE Healthcare). Whole-population Mo were magnetically isolated using anti-CD14 microbeads (Miltenyi Biotec) followed by FACS for proarteriogenic Mo/MΦs (Aria II; BD Biosciences). The following antibodies were used for cell sorting: human anti-TIE2 (R&D Systems), anti-CD14, anti-CD2, anti-CD3, anti-CD19 and anti-CD56 antibodies (BD Biosciences) (Supplemental Table 10).