Cells were collected and washed twice with ice-cold PBS (pH = 8.0); then, DSS crosslinker (Cat#HY-W019543, MedChemExpress) was added to a final concentration at 250 μM, incubated the mixture for 30 min at 37 °C, and then the reaction was quenched with 10 mM Tri-Hcl (pH = 7.5) for 15 min at room temperature. Cells were collected with centrifugation and lysed as above.
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