Simultaneous Detection of Phosphorylated Signaling Molecules

Eighteen well-characterized phosphorylated or cleaved signaling molecules were simultaneously detected using 50 µg kidney proteins by a slide-based PathScan^® Intracellular Signaling Array Kit (#7323, Cell Signaling Technology) according to the manufacturer’s instructions. Images were acquired by briefly exposing the slide to the Chemi Doc XRS+ system (Bio-Rad), and semi-quantitatively analyzed by scanning volume density using Alpha View Software 3.3 (Cell Biosciences, Santa Clara, USA) (33 (link)).

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Zhang Y., Wu Y., Wang W., Liu F., Zhang Y., Yang C., Liu A., Wu J., Zhu T., Nicholson M.L., Fan Y, & Yang B. (2021). Long-Term Protection of CHBP Against Combinational Renal Injury Induced by Both Ischemia–Reperfusion and Cyclosporine A in Mice. Frontiers in Immunology, 12, 697751.

Publication 2021

PathScan® Intracellular Signaling Array Kit Chemi Doc XRS+ system Alpha View Software 3.3

Cell Intracellular signaling proteins Kidney

Corresponding Organization : University of Leicester

Other organizations : Fudan University, Zhongshan Hospital, Addenbrooke's Hospital, University of Cambridge

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Variable analysis

independent variables

Eighteen well-characterized phosphorylated or cleaved signaling molecules

dependent variables

Simultaneous detection of eighteen well-characterized phosphorylated or cleaved signaling molecules

control variables

Kidney proteins (50 µg)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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