Measurement of Serum Metabolites in Offspring

Serum NEFA, TG, and T-Chol levels were measured using NEFA C-Test Wako (#279–75,401), TG E-Test Wako (#432–40,210), and T-Chol E-Test Wako (#439–17,501) kits (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively^{19 (link),22 (link)}. Blood glucose was measured using a glucometer (Glutest PRO R; Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, Japan)^{22 (link)}. As milk samples, we used the gastric contents of offspring, which mainly consisted of milk derived from dams, on d16^{22 (link)}.

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Kawahori K., Kondo Y., Yuan X., Kawasaki Y., Hanzawa N., Tsujimoto K., Wada F., Kohda T., Ishigami A., Yamada T., Ogawa Y, & Hashimoto K. (2020). Ascorbic acid during the suckling period is required for proper DNA demethylation in the liver. Scientific Reports, 10, 21228.

Publication 2020

NEFA C-Test Wako TG E-Test Wako T-Chol E-Test Wako

Blood glucose Gastric contents Milk Nefa Serum

Corresponding Organization : Tokyo Medical and Dental University

Other organizations : Tokyo Metropolitan Institute of Gerontology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum NEFA levels
Serum TG levels
Serum T-Chol levels
Blood glucose levels

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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