Hippocampal RNA Isolation and Analysis

After the MWM, the mice were euthanized via cervical dislocation, and the obtained samples were preserved in liquid nitrogen. The total RNA of the hippocampus was isolated with TRIzol reagent (Invitrogen, CA, United States) according to the previous protocol (Zhao et al., 2022 (link)). The RNA sample used in this study was the same cohort as our reported investigation (Cai et al., 2022 (link)). Subsequently, the RNA integrity was determined using gel electrophoresis (Supplementary Figure 1). The RNA concentration was determined on Spark 20 M microplate reader (Tecan Group Ltd., Switzerland; Supplementary Table 1).

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Sun T., Zeng L., Cai Z., Liu Q., Li Z, & Liu R. (2022). Comprehensive analysis of dysregulated circular RNAs and construction of a ceRNA network involved in the pathology of Alzheimer’s disease in a 5 × FAD mouse model. Frontiers in Aging Neuroscience, 14, 1020699.

Publication 2022

TRIzol reagent Spark 20 M microplate reader

Cervical Dislocation Electrophoresis Hippocampus Mice Nitrogen Trizol

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

Other organizations : Minzu University of China

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

RNA integrity
RNA concentration

control variables

Cervical dislocation for euthanasia
Preservation of samples in liquid nitrogen
Isolation of total RNA from the hippocampus using TRIzol reagent
Use of the same RNA sample cohort as a previous investigation (Cai et al., 2022)

controls

Positive control: None mentioned
Negative control: None mentioned

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