Intracellular Calcium Signaling Modulators

Reagents included ammonium iron(III) citrate (FAC) (Sigma Aldrich, F5879), 2,2-bipyridyl (Sigma Aldrich, 366-18-7), 4,4-bipyridyl (Sigma Aldrich, 533-26-4) and BAPTA-AM (Invitrogen, B6769). Trans-Ned19 (Ned19) and a cell-permeant version of NAADP (NAADP-AM) were synthesized as previously described [24 (link), 25 (link)] and used at a final concentration of 1 μM (Ned19) or 100 nM (NAADP-AM) for 12 h. The TRPML compounds ML-SA1, MK6-83, EVP21, and EVP22 have been previously described [26 (link)–29 ] and were employed at a final concentration of 5 μM for 12 h.

Free full text: Click here

Fernández B., Olmedo P., Gil F., Fdez E., Naaldijk Y., Rivero-Ríos P., Bracher F., Grimm C., Churchill G.C, & Hilfiker S. (2022). Iron-induced cytotoxicity mediated by endolysosomal TRPML1 channels is reverted by TFEB. Cell Death & Disease, 13(12), 1047.

Publication 2022

BAPTA-AM

Ammonium Bapta Bipyridyl Cell Citrate Iron Naadp

Corresponding Organization :

Other organizations : Consejo Superior de Investigaciones Científicas, Instituto de Parasitología y Biomedicina "López - Neyra", Universidad de Granada, Rutgers, The State University of New Jersey, University of Michigan–Ann Arbor, Ludwig-Maximilians-Universität München, University of Oxford

Top 5 similar protocols

Variable analysis

independent variables

Ned19 (concentration: 1 μM, duration: 12 h)
NAADP-AM (concentration: 100 nM, duration: 12 h)
ML-SA1 (concentration: 5 μM, duration: 12 h)
MK6-83 (concentration: 5 μM, duration: 12 h)
EVP21 (concentration: 5 μM, duration: 12 h)
EVP22 (concentration: 5 μM, duration: 12 h)

dependent variables

Not explicitly mentioned

control variables

Ammonium iron(III) citrate (FAC)
2,2-bipyridyl
4,4-bipyridyl
BAPTA-AM

positive controls

None mentioned

negative controls

None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!