Immunoblot Analysis of Apoptosis Regulators

Whole cell lysates were prepared and subjected to IB analysis using antibodies against SAG (monoclonal antibody raised againt the RING domain (AA44-113), Bax, Bad, cIAP2, (Cell signaling), pRB, Puma, Mcl-1 (Santa Cruz Biotechnology), XIAP, Bcl-XL, p21, (BD Transduction Laboratories), β-Actin (Sigma), Bak (Upstate), Bim (Imgenex), Noxa (Oncogene Science), Bcl-2, (Dako), and survivin (Novus biologicals).

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Jia L., Yang J., Hao X., Zheng M., He H., Xiong X., Xu L, & Sun Y. (2010). Validation of SAG/RBX2/ROC2 E3 ubiquitin ligase as an anti-cancer and radio-sensitizing target. Clinical cancer research : an official journal of the American Association for Cancer Research, 16(3), 814.

Publication 2009

Actin Antibodies Bcl 2 Biologicals Cell Ciap2 Monoclonal antibody Novus Oncogene Puma Survivin

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Whole cell lysates were prepared

dependent variables

IB analysis using antibodies against SAG (monoclonal antibody raised againt the RING domain (AA44-113), Bax, Bad, cIAP2, (Cell signaling), pRB, Puma, Mcl-1 (Santa Cruz Biotechnology), XIAP, Bcl-XL, p21, (BD Transduction Laboratories), β-Actin (Sigma), Bak (Upstate), Bim (Imgenex), Noxa (Oncogene Science), Bcl-2, (Dako), and survivin (Novus biologicals)

control variables

No control variables were explicitly mentioned.

controls

No positive or negative controls were explicitly mentioned.

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