After 21 days, xylazine hydrochloride (Rompun, Bayer,
Germany; 10 mg/kg) and ketamine hydrochloride (Ketalar,
Bayer, Germany; 40 mg/kg) were used for anesthesia. After
the cardiac blood samples were collected from the heart,
the animals were sacrificed by guillotine method. Mandibula
samples were removed from the heads. The mandibles
were divided into two equal pieces (16 (link)). The right sides
were forwarded to the histology laboratory for histological
evaluation and 10% neutral-buffered formaldehyde solution
was used for fixation. The left sides of the mandibula were sent
to the biochemistry laboratory for biochemical analysis. The
blood samples were centrifuged at 3500 xg for 15 minutes to
obtain serum. Sterile polypropylene tubes were used to save serum samples and kept at -80°C until being analyzed for
MMP-8 and MMP-13 (1 (link)).
Germany; 10 mg/kg) and ketamine hydrochloride (Ketalar,
Bayer, Germany; 40 mg/kg) were used for anesthesia. After
the cardiac blood samples were collected from the heart,
the animals were sacrificed by guillotine method. Mandibula
samples were removed from the heads. The mandibles
were divided into two equal pieces (16 (link)). The right sides
were forwarded to the histology laboratory for histological
evaluation and 10% neutral-buffered formaldehyde solution
was used for fixation. The left sides of the mandibula were sent
to the biochemistry laboratory for biochemical analysis. The
blood samples were centrifuged at 3500 xg for 15 minutes to
obtain serum. Sterile polypropylene tubes were used to save serum samples and kept at -80°C until being analyzed for
MMP-8 and MMP-13 (1 (link)).
