Red Ginseng UPLC-QToF-MS Analysis

For the UPLC-QToF-MS analysis, the powdered red ginseng samples were extracted in a manner similar to our previous studies using ultrasonic extraction [13 (link)]. In total, 300 mg of each powered red ginseng was weighed in a centrifuge tube (15 mL, PP-single use; BioLogix Group, Jinan, Shandong, China) and shaken vigorously after the addition of 6 mL of methanol. The extract was then placed in an ultrasonic cleaner (60 Hz; Wiseclean, Seoul, Korea) for 30 min. The solution was centrifuged (Legand Mach 1.6R; Thermo, Frankfurt, Germany) at a speed of 3000 rpm for 10 min, and an aliquot of the supernatant solution was filtered (0.2 µm; Acrodisk, Gelman Sciences, Ann Arbor, MI, USA) and injected into the UPLC system (Waters Co., Milford, MA, USA).