Protocol detail

Cell Lysis and Antibody Analysis

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Cells were lysed as previously described [30] (link). The following antibodies that follow were used: anti-Tag (Pab101, [84] (link)), anti-actin (I-19, Santa Cruz), anti-CHK1 (G-4, Santa Cruz), anti-CHK1 pS317 (Cell Signaling), anti-CHK2 pT68 (Y171, Epitomics), anti-CHK2 (EPR4325, Epitomics), anti-NBS1 pS343 (EP178, Epitomics), anti-NBS1 (A301-289A, Bethyl Laboratories), anti-ATR (N-19, Santa Cruz), anti-DNA-PK_cs (G-4, Santa Cruz), anti-KU80 (C-20, Santa Cruz), anti-KU70 (M-19, Santa Cruz), anti-DNA-PK_cs pS2056 (EPR5670, Epitomics), anti-CtIP (A300-488A, Bethyl Laboratories), and anti-GAPDH (0411, Santa Cruz).

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Sowd G.A., Mody D., Eggold J., Cortez D., Friedman K.L, & Fanning E. (2014). SV40 Utilizes ATM Kinase Activity to Prevent Non-homologous End Joining of Broken Viral DNA Replication Products. PLoS Pathogens, 10(12), e1004536.

Publication 2014

anti-Tag anti-actin (I-19, anti-CHK1 (G-4, anti-CHK1 pS317 anti-KU80 (C-20, anti-DNA-PKcs pS2056 anti-GAPDH

A301 Actin Anti dna Antibodies Cells Ctip Dna pkcs Gapdh Ku70 Pkcs

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Variable analysis

independent variables

Cell lysis protocol as previously described [30]

dependent variables

Protein expression levels of the following proteins measured by immunoblotting:
- Tag
- Actin
- CHK1
- Phosphorylated CHK1 (pS317)
- CHK2
- Phosphorylated CHK2 (pT68)
- NBS1
- Phosphorylated NBS1 (pS343)
- ATR
- DNA-PKcs
- KU80
- KU70
- Phosphorylated DNA-PKcs (pS2056)
- CtIP
- GAPDH

control variables

Positive controls: None specified
Negative controls: None specified

Annotations

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