A muscovite mica (grade V1) substrate was subjected to a fresh cleaving process to achieve optimal surface conditions for Atomic Force Microscopy (AFM) imaging. Subsequently, a 200 μL volume of a purified DNA origami sample was combined with 100 μL of a TAE buffer containing 12.5 mM Mg(OAc) 2 . The resultant mixture was gently deposited onto the surface of the mica substrate.
Following deposition, adsorption was allowed to proceed for 35 min at room temperature before initiating the AFM experiment.