Visualizing Pancreatic Endocrine Cells

Pancreatic sections were stained with anti-CD3 (CD3-12, Thermo Fisher), anti-BSRP-A (polyclonal sheep IgG, R&D), and DAPI. Images were obtained using Nikon Ti-E widefield microscope at x20 magnification. BSRP-A was used to identify endocrine cells [50 (link),51 (link)]. Nikon NIS-Elements scope/software was used to obtain the images. Background autofluorescence was removed using a previously published method in Fiji [52 (link)].

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Kong Y., Jing Y., Allard D., Scavuzzo M.A., Sprouse M.L., Borowiak M., Bettini M.L, & Bettini M. (2022). A dormant T cell population with autoimmune potential exhibits low self-reactivity and infiltrates islets in type 1 diabetes. European journal of immunology, 52(7), 1158-1170.

Publication 2022

Ti-E widefield microscope

Anti cd3 Dapi Endocrine cells Microscope Pancreatic Sheep

Corresponding Organization : University of Utah

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Variable analysis

independent variables

Use of anti-CD3 antibody
Use of anti-BSRP-A antibody

dependent variables

Identification of CD3-positive cells
Identification of BSRP-A-positive cells (endocrine cells)

control variables

Pancreatic sections
Staining with DAPI
Imaging using Nikon Ti-E widefield microscope at x20 magnification
Use of Nikon NIS-Elements scope/software
Removal of background autofluorescence using a previously published method in Fiji

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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