Immunohistochemistry Staining Protocol

Immunohistochemical studies were performed on formalin-fixed, paraffin-embedded sections as previously described [19 (link), 40 (link)], or in a Dako AutoStainer (Dako, Carpinteria, CA). Antibodies used include: anti-CD68 (clone PG-M1, Dako, dilution 1:100); anti-CD163 (clone10D6, Biocare Medical, Concord, CA, USA, dilution 1:50); anti-CD31 (clone JC70A, Dako, ready-to use); anti-MMP-9 (clone Ab-2, Oncogene Research Products Cambridge, MA, dilution 1:50); and anti E-cadherin (Dako, clone NCH-38, ready to use).

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Ciucci A., Zannoni G.F., Buttarelli M., Martinelli E., Mascilini F., Petrillo M., Ferrandina G., Scambia G, & Gallo D. (2016). Ovarian low and high grade serous carcinomas: hidden divergent features in the tumor microenvironment. Oncotarget, 7(42), 68033-68043.

Publication 2016

AutoStainer anti-CD68 (clone PG-M1, anti-CD163 (clone10D6, anti-CD31 (clone JC70A, anti E-cadherin

Antibodies Cadherin Cd163 Clone Dilution Embedded paraffin Formalin Mmp 9 Oncogene products

Corresponding Organization : Università Cattolica del Sacro Cuore

Other organizations : University of Molise

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Variable analysis

independent variables

Formalin-fixed, paraffin-embedded sections

dependent variables

Expression of CD68
Expression of CD163
Expression of CD31
Expression of MMP-9
Expression of E-cadherin

control variables

Previously described immunohistochemical studies [19, 40]
Dako AutoStainer (Dako, Carpinteria, CA)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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