Flow cytometry was performed as previously described with modification (Barletta et al., 2012 (link)). The neutrophils were stained with 0.1 μg of FITC-mouse Ly6G antibody (eBioscience, San Diego, CA, USA) and 0.1 μg of PE-mouse CD11b antibody (eBioscience). All of the experiments were recorded using Accuri Cflow software (BD Bioscience, CA, USA) and were analyzed using FlowJo software (Three Star, Ashland, OR, USA).
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