Blood samples were collected from the mice under isoflurane anesthesia at autopsy (43
weeks of age). Anti-dsDNA antibodies, BUN, and total cholesterol levels were determined
using a mouse anti-dsDNA enzyme-linked immunosorbent assay (ELISA) kit (Shibayagi Co.,
Ltd., Ishihara, Shibukawa, Japan), a urea nitrogen colorimetric detection kit (EIABUN;
Invitrogen, Carlsbad, CA, USA), and a total cholesterol colorimetric assay kit
(MBS2540484, Mybiosource, San Diego, CA, USA), as described in our previous study [8 (link)].
weeks of age). Anti-dsDNA antibodies, BUN, and total cholesterol levels were determined
using a mouse anti-dsDNA enzyme-linked immunosorbent assay (ELISA) kit (Shibayagi Co.,
Ltd., Ishihara, Shibukawa, Japan), a urea nitrogen colorimetric detection kit (EIABUN;
Invitrogen, Carlsbad, CA, USA), and a total cholesterol colorimetric assay kit
(MBS2540484, Mybiosource, San Diego, CA, USA), as described in our previous study [8 (link)].
