Yeast tRNAPhe was purchased from Sigma-Aldrich. Other isoacceptor tRNAs were prepared from bulk tRNA (Roche) from either E. coli (tRNAVal, tRNALys, tRNAGln), and or S. cerevisiae (tRNAArg), via hybridization to immobilized complementary oligo DNAs as described previously49 (link),50 (link). E. coli and yeast tRNAs were charged with their cognate amino acids as described21 (link),27 (link),46 (link).
Free full text: Click here