Protocol detail

Microcomputed Tomography Analysis of Trabecular Bone

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Each specimen was placed on the scanning platform of a GE eXplore Locus µCT (GE Healthcare, Piscataway, NJ) and 360 X-ray projections were collected (80 kVp; 500 mA; 26 min total scan time). Projection images were preprocessed and reconstructed into 3D volumes (20 µm resolution) on a 4PC reconstruction cluster using a modified tent-FDK cone beam algorithm (GE reconstruction software). The 3D data were processed and rendered (isosurface/maximum intensity projections) using MicroView (GE Healthcare). Trabecular bone volume in a defect site was calculated using image analysis of µCT data (MicroView, GE Healthcare). Briefly, after 3D reconstruction, each volume was scaled to Hounsfield Units (HU) using a calibration phantom containing air and water (phantom plastic); a plug within the phantom containing hydroxyapatite was used as a bone mimic for bone mineral/density calculations. Volumes were imported into Matlab (R2009b, Mathworks) for automated batch analysis [16 (link)]. Trabecular bone volume (BV) was divided by the ROI volume (total volume, TV) in order to calculate BV/TV%.

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Kim J., Magno M.H., Ortiz O., McBride S., Darr A., Kohn J, & Hollinger J.O. (2015). Next-generation resorbable polymer scaffolds with surface-precipitated calcium phosphate coatings. Regenerative Biomaterials, 2(1), 1-8.

Publication 2015

MicroView Matlab

Bone Bone mineral density Cone Hydroxyapatite Reconstruction Scan Trabecular bone X ray

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Variable analysis

independent variables

X-ray projection collection (80 kVp; 500 mA; 26 min total scan time)
3D volume reconstruction (20 µm resolution)

dependent variables

Trabecular bone volume in a defect site
Bone mineral/density calculations

control variables

Scanning platform of a GE eXplore Locus µCT
Calibration phantom containing air, water, and hydroxyapatite

controls

Calibration phantom containing air and water (phantom plastic)
Hydroxyapatite plug within the phantom as a bone mimic for bone mineral/density calculations

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