Western Blot Analysis of TMEM16A Protein

Proteins from the cell lysates were prepared from cell lines as previously described [16 (link)]. Equal amounts of protein were denatured and separated by SDS-PAGE, transferred onto PVDF membranes, and incubated with polyclonal antibodies to TMEM16A (Abcam, ab53212; 1:100) and mouse β-actin antibody (Cell Signalling; 1:500). The peak intensity of each band was visualized using an Enhanced Chemiluminescence kit (Amersham, Little Chalfont, UK) on X-ray film (Millipore Corporation, Billerica, USA).

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Sui Y., Wu F., Lv J., Li H., Li X., Du Z., Sun M., Zheng Y., Yang L., Zhong L., Zhang X, & Zhang G. (2015). Identification of the Novel TMEM16A Inhibitor Dehydroandrographolide and Its Anticancer Activity on SW620 Cells. PLoS ONE, 10(12), e0144715.

Publication 2015

ab53212 mouse β-actin antibody Enhanced Chemiluminescence kit X-ray film

Actin Antibodies Antibody Cell Cell lines Chemiluminescence Membranes Mouse Protein Pvdf Sds page X ray film

Corresponding Organization : First Bethune Hospital of Jilin University

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Variable analysis

independent variables

Preparation of proteins from cell lysates

dependent variables

Peak intensity of each band

control variables

Equal amounts of protein

controls

Positive control: Not mentioned
Negative control: Not mentioned

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