Immunofluorescence Microscopy of Treated Cells

Immunofluorescence microscopy was carried out as described^{67 (link)}. HeLa or U2OS cells were treated with the indicated compounds for 4 hours, fixed, permeabilized, and co-stained with Hoechst 33342 (DNA stain) and the indicated antibodies. Images were captured with a Leica DMI6000 microscope (Leica DFC360 FX Camera, 63x/1.40-0.60 NA oil objective, Leica AF6000 software). Images were deconvolved with Leica Application Suite 3D Deconvolution software and exported as TIFF files.

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Lo Y.C., Senese S., France B., Gholkar A.A., Damoiseaux R, & Torres J.Z. (2017). Computational Cell Cycle Profiling of Cancer Cells for Prioritizing FDA-Approved Drugs with Repurposing Potential. Scientific Reports, 7, 11261.

Publication 2017

DFC360 FX Camera Application Suite 3D Deconvolution software

Antibodies Cells Hela Hoechst 33342 Immunofluorescence microscopy Microscope Stain

Corresponding Organization : University of California, Los Angeles

Other organizations : Los Angeles Medical Center

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Variable analysis

independent variables

The indicated compounds

dependent variables

Cellular localization and intensity of the indicated antibodies

control variables

Hoechst 33342 (DNA stain)
Leica DMI6000 microscope (Leica DFC360 FX Camera, 63x/1.40-0.60 NA oil objective, Leica AF6000 software)
Leica Application Suite 3D Deconvolution software

controls

No positive or negative controls were explicitly mentioned in the provided information.

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