Western Blotting Protein Analysis

Western blotting was performed as described (58 (link)). The following primary antibodies were used: mouse anti-V5 (1:5,000; Thermo Fisher Scientific, Waltham, MA, USA), mouse anti-tubulin (1:2,000; Solarbio, Beijing, China), rabbit Anti-GAPDH (1:2,000; Solarbio), and rabbit anti-Hypusine (EMD Millipore; 1:1,000) antibodies, rabbit anti-eIF5A (1:1,000; ABclonal), rabbit anti-DHPS (1:200; abcam), rabbit anti-DOHH (1:500; Sigma-Aldrich). Secondary antibodies used were Goat Anti-Mouse horseradish peroxidase-conjugated secondary antibody (1:2,000; Beyotime, Shanghai, China) and Goat Anti-Rabbit horseradish peroxidase-conjugated secondary antibody (1:5,000). Anti-His (M1001020, Solarbio), Anti-pAKT473 (GB13012-3, Servicebio), Anti-pAKT308 (ab66134, Abcam), Anti-ATPaseβ (GL Binchem synthesis), Anti-Inx1(GL Binchem synthesis), Anti-Inx2 (GL Binchem synthesis), Anti-Inx3(GL Binchem synthesis), Anti-Inx4 (GL Binchem synthesis), Cleaved-caspase (WL02117, Wanleibio), Anti-GAPDH (M1000110, Solarbio), Anti-β Tubulin (AF1216, Beyotime), Goat Anti-Mouse (A0216, Beyotime), Goat Anti-Rabbit (A0208, Beyotime). Proteins were semi-quantified via densitometry using ImageJ (National Institutes of Health, Bethesda, MD, USA).