Venous Blood Sampling for Lipid and DNA Analysis

Venous blood samples of 5 mL were drawn after at least 12 h of fasting. Two-fifths of the sample (2 mL) was collected in glass tubes and used to determine serum lipid levels. The remaining three-fifths of the sample (3 mL) was transferred to tubes with anticoagulants (4.80 g/l citric acid, 14.70 g/l glucose and 13.20 g/l trisodium citrate) and used to extract DNA. Measurements of serum TC, TAG, HDL-C, and LDL-C levels in the samples were performed by enzymatic methods with commercially available kits (RANDOX Laboratories Ltd., BT29 4QY; Daiichi Pure Chemicals Co., Ltd.). Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay using a commercial kit (RANDOX Laboratories Ltd.). All determinations were performed with an auto-analyser (Type 7170A; Hitachi Ltd.) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [15 (link)–17 (link)].

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Guo T., Yin R.X., Lin Q.Z., Wu J., Shen S.W., Sun J.Q., Shi G.Y., Wu J.Z., Li H, & Wang Y.M. (2014). Polymorphism of rs873308 near the transmembrane protein 57 gene is associated with serum lipid levels. Bioscience Reports, 34(2), e00095.

Publication 2014

Type 7170A

Anticoagulants Apoa1 Apob Blood Citric acid Enzymatic Glucose Immunoassay Immunoturbidimetric Lipid Randox Serum Trisodium citrate Venous

Corresponding Organization :

Other organizations : Guangxi Medical University

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Variable analysis

independent variables

Fasting duration (at least 12 h)

dependent variables

Serum total cholesterol (TC) levels
Serum triglyceride (TAG) levels
Serum high-density lipoprotein cholesterol (HDL-C) levels
Serum low-density lipoprotein cholesterol (LDL-C) levels
Serum apolipoprotein A1 (ApoA1) levels
Serum apolipoprotein B (ApoB) levels

control variables

Volume of venous blood sample (5 mL)
Anticoagulants used in the tubes (4.80 g/l citric acid, 14.70 g/l glucose, and 13.20 g/l trisodium citrate)
Enzymatic methods and commercial kits used for lipid and apolipoprotein measurements
Auto-analyzer (Type 7170A; Hitachi Ltd.) used for all determinations

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