Immunofluorescence Analysis of Key Cellular Markers

Immunofluorescence was performed as described previously (13 (link)). The following primary antibodies were used: anti-NFκB (GTX102090, GeneTex), anti-β-catenin (E247, Epitomics #1247-s), anti-E-cadherin (GTX100443, GeneTex), and anti-N-cadherin (TA326835, OriGene). Goat anti-rabbit secondary antibody was coupled to AlexaFluor 555 (Invitrogen). Images were obtained with DS-Ri1 Nikon camera and Eclipse80i Nikon microscope and quantifications were performed using NIS-Elements BR 4.20.00 software (Nikon).

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Chamard-Jovenin C., Thiebaut C., Chesnel A., Bresso E., Morel C., Smail-Tabbone M., Devignes M.D., Boukhobza T, & Dumond H. (2017). Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells. Frontiers in Endocrinology, 8, 272.

Publication 2017

GTX102090 GTX100443 AlexaFluor 555 DS-Ri1 Nikon camera Eclipse80i Nikon microscope NIS-Elements BR 4.20.00 software

Anti antibody Antibodies Cadherin Goat Immunofluorescence Microscope Nfκb Rabbit β catenin

Corresponding Organization : Centre de Recherche en Automatique de Nancy

Other organizations : Laboratoire Lorrain de Recherche en Informatique et ses Applications, Centre Inria de l'Université de Lorraine

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Variable analysis

independent variables

Primary antibodies used: anti-NFκB, anti-β-catenin, anti-E-cadherin, anti-N-cadherin

dependent variables

Localization and expression of NFκB, β-catenin, E-cadherin, and N-cadherin proteins

control variables

Immunofluorescence protocol was performed as described previously (13)
Goat anti-rabbit secondary antibody coupled to AlexaFluor 555 was used
Images were obtained with DS-Ri1 Nikon camera and Eclipse80i Nikon microscope
Quantifications were performed using NIS-Elements BR 4.20.00 software (Nikon)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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