Total protein extraction, SDS PAGE, and Western blot analysis were performed as previously described [54 (link)]. The following primary antibodies were used: pMEK1/2 (phosphor-MEK1/2 Ser217/221) #9121, MEK1/2 (L38C12) #4696, pERK1/2 (phospho-ERK1/2 Thr202/Tyr204) #9101, ERK1/2 (L34F12) #4696 from Cell Signaling Technology Inc (Danvers, MA, USA); Vinculin #V9131 from Sigma-Aldrich was included as protein loading control.
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