Quantitative Metabolomics in Diverse Tissues

For the quantitative demo data, we extracted intracellular metabolites from 5 different tissues (brain, liver, kidney, spleen and pancreas) from a ¹³C-tracing experiment with ¹³C-methanol in four different mice [8 (link)]. Metabolite extraction and LC-MS measurement were performed as described in [9 (link)]. Only the unlabelled metabolites are used here, to show the differences between the tissues. Peak areas were extracted using TraceFinder-software (Thermo Fisher), by comparing the mass to charge ratio (m/z) and the retention time against a custom library prepared with authentic standards. The dataset contains 99 metabolites measured for 3 time-points in 4 biological replicates.