Immunoblotting Antibody Dilution Protocol

Mouse anti-SCAR (DSHB P1C1-SCAR, RRID: AB_2618386) was used at a dilution 1:250. Rat anti HA-HRP (Roche catalog number: 12013819001, RRID:AB_390917) was used at a dilution of 1:500. Guinea Pig anti-Non-stop (Mohan et al., 2014a (link)) was used at a dilution of 1:1000. Guinea Pig anti-Ada2b (Kusch et al., 2003 (link)) was used at a dilution of 1:1000. Rabbit anti-Atxn7 (Mohan et al., 2014a (link)) was used at a concentration of 1:2000. Goat anti Guinea Pig HRP (Jackson ImmunoResearch INC catalog number: 106-035-003, RRID:AB_2337402) was used at a dilution of 1:10,000. Goat anti-mouse HRP (Jackson ImmunoResearch INC catalog number: 115-035-003, RRID:AB_10015289) was used at a dilution of 1:5000. Goat anti-Rabbit HRP (Jackson ImmunoResearch INC catalog number: 111-035-003, RRID:AB_2313567) was used at a dilution of 1:10,000.

Free full text: Click here

Cloud V., Thapa A., Morales-Sosa P., Miller T.M., Miller S.A., Holsapple D., Gerhart P.M., Momtahan E., Jack J.L., Leiva E., Rapp S.R., Shelton L.G., Pierce R.A., Martin-Brown S., Florens L., Washburn M.P, & Mohan R.D. (2019). Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization. eLife, 8, e49677.

Publication 2019

Rat anti HA-HRP Goat anti Guinea Pig HRP Goat anti-mouse HRP Goat anti-Rabbit HRP

Dilution Goat Guinea pig Mouse Rabbit Scar

Corresponding Organization :

Other organizations : University of Missouri–Kansas City, Stowers Institute for Medical Research, University of Kansas Medical Center

Top 5 similar protocols

Variable analysis

independent variables

Mouse anti-SCAR (DSHB P1C1-SCAR, RRID: AB_2618386) was used at a dilution 1:250
Rat anti HA-HRP (Roche catalog number: 12013819001, RRID:AB_390917) was used at a dilution of 1:500
Guinea Pig anti-Non-stop (Mohan et al., 2014a) was used at a dilution of 1:1000
Guinea Pig anti-Ada2b (Kusch et al., 2003) was used at a dilution of 1:1000
Rabbit anti-Atxn7 (Mohan et al., 2014a) was used at a concentration of 1:2000
Goat anti Guinea Pig HRP (Jackson ImmunoResearch INC catalog number: 106-035-003, RRID:AB_2337402) was used at a dilution of 1:10,000
Goat anti-mouse HRP (Jackson ImmunoResearch INC catalog number: 115-035-003, RRID:AB_10015289) was used at a dilution of 1:5000
Goat anti-Rabbit HRP (Jackson ImmunoResearch INC catalog number: 111-035-003, RRID:AB_2313567) was used at a dilution of 1:10,000

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!