The cell lines SW480 and RKO were seeded in 12-well plates with one coverslip per well. 24 h after seeding, the treatments were processed as described in Section 2.6. 48 h later the cells were fixed and stained as previously reported by us [36 (link)]. Representative images were obtained using a fluorescence microscope (Olympus motorized BX63F Upright Microscope) at a magnification of 600×.
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