Cell Line Maintenance and Testing

HT1080 cells were obtained from ATCC and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (catalog number 11995065; Thermo Fisher) with 10% fetal calf serum (FCS) (catalog number F8067-500ML; Sigma) and gentamicin (catalog number 15710064; Thermo Fisher). An IFN-α (catalog number 11200-2; PBL Assay Science)-sensitive HT1080 single-cell clone was derived by limiting dilution. BHK21 cells obtained from ATCC were maintained in Eagle’s minimum essential medium (EMEM) (catalog number 30-2003; ATCC) supplemented with 10% FCS (catalog number F8067-500ML; Sigma) and gentamicin (catalog number 15710064; Thermo Fisher). Cells were not tested for mycoplasma contamination but were periodically tested for retrovirus contamination using a PCR-based reverse transcriptase assay (35 (link), 36 (link)).
BSRT7 cells (a kind gift from K. Conzelmann) (37 (link)) and African green monkey kidney Vero cells (CCL-81; ATCC) were maintained in DMEM (catalog number 10-013-CV; Corning, Inc.) containing 10% fetal bovine serum (FBS) (catalog number TCB 101; Tissue Culture Biologicals) at 37°C and 5% CO₂.

Free full text: Click here

Kueck T., Bloyet L.M., Cassella E., Zang T., Schmidt F., Brusic V., Tekes G., Pornillos O., Whelan S.P, & Bieniasz P.D. (2019). Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. Journal of Virology, 93(24), e01372-19.

Publication 2019

DMEM gentamicin IFN-α

African green monkey Assay Biologicals Cell clone Cells Dilution Eagle Fetal bovine serum Gentamicin Ifn α Kidney Mycoplasma Retrovirus Reverse transcriptase Tissue Vero cells

Corresponding Organization :

Other organizations : Rockefeller University, Harvard University, Howard Hughes Medical Institute, University of Virginia

Top 5 similar protocols

Variable analysis

independent variables

Source of cell lines (HT1080, BHK21, BSRT7, Vero)
Treatment with IFN-α

dependent variables

Retrovirus contamination

control variables

Cell culture media (DMEM, EMEM)
Serum supplementation (10% FCS/FBS)
Antibiotic (gentamicin)
Incubation conditions (37°C, 5% CO2)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!