Transgenic Zebrafish Strains for Live Imaging

Transgenic zebrafish strains used in this study were Tg(fli1a:EGFP)^y1 [28 (link)], Tg(fli1a:H2B-mCherry)^uq37bh [43 (link)], and Tg(fli1aep:ERK-KTR-Clover)^uq39bh [32 (link)]. Larvae/embryos were anaesthetised in 0.08 mg/mL tricaine and mounted as previously described [44 (link)]. Zebrafish live-imaging was conducted using either an Olympus MVX fluorescent microscope, Zeiss LSM 710 FCS confocal microscope, Zeiss Elyra 780 confocal microscope, Nikon Yokogawa CSU-W1 spinning disc confocal microscope, or the Zeiss Z. 1 lightsheet fluorescent microscope.

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Ng M.F., Da Silva Viana J., Tan P.J., Britto D.D., Choi S.B., Kobayashi S., Samat N., Song D.S., Ogawa S., Parhar I.S., Astin J.W., Hogan B.M., Patel V, & Okuda K.S. (2024). Canthin-6-One Inhibits Developmental and Tumour-Associated Angiogenesis in Zebrafish. Pharmaceuticals, 17(1), 108.

Publication 2024

LSM 710 FCS confocal microscope

Corresponding Organization :

Other organizations : Cancer Research Malaysia, Peter MacCallum Cancer Centre, University of Melbourne, University of Auckland, UCSI University, Monash University Malaysia, University of Queensland, La Trobe University

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Variable analysis

independent variables

Transgenic zebrafish strains used in this study: Tg(fli1a:EGFP)y1, Tg(fli1a:H2B-mCherry)uq37bh, and Tg(fli1aep:ERK-KTR-Clover)uq39bh

dependent variables

Observations made during zebrafish live-imaging using various microscopes: Olympus MVX fluorescent microscope, Zeiss LSM 710 FCS confocal microscope, Zeiss Elyra 780 confocal microscope, Nikon Yokogawa CSU-W1 spinning disc confocal microscope, and Zeiss Z. 1 lightsheet fluorescent microscope

control variables

Larvae/embryos were anaesthetised in 0.08 mg/mL tricaine and mounted as previously described [44 (link)]

controls

No positive or negative controls were explicitly mentioned in the provided information.

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