Plasma concentrations of cytokines, chemokines, and growth factors were determined using a Bio-plex Multiplex Immunoassay System (Bio-Rad, Hercules, CA, USA) using a Bio-plex ProTM Human Cytokine 27-plex assay kit as reported previously [12 (link), 13 (link)] as per the manufacturer’s instructions. Levels of 15 cytokines, including the pro-inflammatory (IL-1β, IL-5, IL-6, IL-7, IL-9, IL-15, IL-17, TNF-α), anti-inflammatory (IL1-RA, IL-4, IL-10, IL-13), and Th1 (IL-2, IFN-γ, IL-12 p70) cytokines along with seven chemokines (eotaxin, CCL-2,CCL-3,CCL-4,CCL-5, IL-8, CXCL-10) and five growth factors (basic fibroblast growth factor [FGF], G-CSF, GM-CSF, vascular endothelial growth factor [VEGF], platelet-derived growth factor-bb [PDGF-bb]), were estimated.
Protocol detail
Multiplex Profiling of Plasma Biomarkers
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Anti inflammatory
Assay
Basic fibroblast growth factor
Chemokines
Cytokines
Eotaxin
G csf
Gm csf
Growth factors
Human
Ifn γ
Il 10
Il 12 p70
Il 13
Il 15
Il 17
Il 1β
Immunoassay
Inflammatory
Plasma
Platelet derived growth factor bb
Tnf α
Vascular endothelial growth factor
Corresponding Organization : ICMR-National Institute of Virology
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Variable analysis
independent variables
- Not explicitly mentioned
- Plasma concentrations of cytokines
- Plasma concentrations of chemokines
- Plasma concentrations of growth factors
- Not explicitly mentioned
- Positive control: Not mentioned
- Negative control: Not mentioned
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