Craniofacial Phenotyping of Acp4 Mutant Mice

Frontal facial images were taken of 7-week Acp4^+/+, Acp4^+/R110C, and Acp4^R110C/R110C mice lightly anesthetized with isoflurane using the Nikon SMZ1000 dissection microscope with a Nikon DXM1200 digital camera. Three samples from each genotype were evaluated. Separately, another group of mice were deeply anesthetized with isoflurane and perfused with 1× phosphate buffered saline (PBS) for 10 min. Mandibles were dissected out and cleaned of soft tissues, post-fixed by immersion in 4% paraformaldehyde (PFA) overnight, then rinsed with PBS three times for 5 min each. Hemimandibles were cleaned with 1% bleach (sodium hypochlorite), rinsed with PBS, air dried, displayed on the dissection microscope, and photographed, as described previously^{50 (link)}.