GC-MS Metabolomics Analysis of Soil Samples

The soil sample for GC-MS metabolomics analysis was prepared as described in our recent publication [3 (link)]. The reliability of the entire analysis was justified by inserting one quality control (QC) sample among every 10 samples. The QC sample was prepared by mixing the extract of all samples in equal volume, and the volume of each QC sample was the same as that of the tested sample. Furthermore, all soil samples were stored at room temperature for GC-MS metabolomics analysis, which was carried out and analyzed as described in our recent publication on a 7890B-5977A GC/MSD GC-MS (Agilent Technologies Inc., Santa Clara, CA, USA) [3 (link)]. The obtained data in this study were analyzed by comparing them with that of the standard spectrum library of the National Institute of Standards and Technology, while the obtained metabolite information in this study was searched against the KEGG database.