Cytokine Profile in HIV Transmission

Aliquots of CVLs from various study visits in Segments A and B (see Fig 2 for exact time points) were assayed by Luminex (Bio-Plex MAGPIX, Bio-Rad Laboratories, Hercules, California, USA) for cytokines that have been associated with HIV transmission [33 (link)–38 (link)], utilizing a custom Invitrogen ProcartaPlex multiplex kit (ThermoFisher, Waltham, Massachusetts, USA) per manufacturer’s protocol. The analytes included: TNF-1α, IL-6, IL-1α, IL-1β, IL-1RA, MIP-1α, MIP-1β, RANTES, SDF-1α, IL-8, MCP-1, IP-10, GM-CSF, IFN-γ, IL-10, and IL-12p40.

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Politch J.A., Cu-Uvin S., Moench T.R., Tashima K.T., Marathe J.G., Guthrie K.M., Cabral H., Nyhuis T., Brennan M., Zeitlin L., Spiegel H.M., Mayer K.H., Whaley K.J, & Anderson D.J. (2021). Safety, acceptability, and pharmacokinetics of a monoclonal antibody-based vaginal multipurpose prevention film (MB66): A Phase I randomized trial. PLoS Medicine, 18(2), e1003495.

Publication 2021

Bio-Plex MAGPIX ProcartaPlex multiplex kit

Cytokines Gm csf Ifn γ Il 10 Il 12p40 Il 1ra Il 1β Mcp 1 Rantes Transmission

Corresponding Organization : Harvard University

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Variable analysis

independent variables

Study visits in Segments A and B

dependent variables

Cytokines: TNF-1α, IL-6, IL-1α, IL-1β, IL-1RA, MIP-1α, MIP-1β, RANTES, SDF-1α, IL-8, MCP-1, IP-10, GM-CSF, IFN-γ, IL-10, and IL-12p40

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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