Post-full-scan MS and (all ion & target) MS/MS data sets were analyzed by Agilent MassHunter Qualitative Analysis (version B.10.00) using a self-built PCDL library that contained known or hypothetical chemical formula for phytochemical components (mainly lignans) and corresponding metabolites. Molecular feature extraction (MFE) and find by formula (FBF) parameters were as follows: mass error <10 ppm, absolute peak area >5000 counts, maximum number of matches 5, and chromatogram extraction window 20 ppm. The criteria for metabolites screened for further evaluation included mass error for protonated molecule being less than 5 ppm, consistent isotopic pattern, retention time plausible for the proposed structure et al. By removing metabolites with the maximum intensity in t0, removing metabolites that are only present in one experiment or one-time point sample, removing metabolites whose peak area appears to be constant across the experiment (the threshold reaction speed ratio = 3), removing metabolites that are present in the experiment but at randomly elevated concentrations (the threshold fluctuation ratio = 10), etc. method to remove false positive metabolites.