Thermo Fisher Scientific supplied mouse anti-E-cadherin, mouse anti-vimentin, mouse anti-catenin, mouse anti-galectin, mouse anti-PD-L1, mouse anti-TGF, mouse anti-NF-κB antibodies, mouse anti-STAT3, and Hoechst (Waltham, MA, USA). eBio-science provided mouse anti-CD163-PerCP, mouse anti-CD206-PerCP, mouse anti-Ki-67-APC, mouse anti-CD68-FITC, and mouse anti-IL-10-FITC (San Diego, CA, USA). BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit with GolgiPlug™(No. BDB555028, San Diego, CA, USA). PeproTech delivered recombinant mouse IL-4, IFN-, and anti-IL-10 receptor (abIL-10R) inhibitors (Rocky Hill, NJ, USA).
mouse anti-STAT3 (Invitrogen, cat number MA1-13042), rabbit anti-NF-κB (Life technologies, cat number 510500), mouse anti-PD-L1/CD274 (Proteintech, cat number 66248-1-Ig), and mouse anti-TGF (Invitrogen, cat number MA5-15065) were used for immunoblotting, and so were secondary antibodies goat anti-mouse and goat anti-rabbit Alexa® Fluor 555 (Thermo Fisher Scientific, Waltham). For immunoblotting, rabbit anti-actin (LI-COR Biosciences, Lincoln, Nebraska, USA) was used. The anti-mouse PD-L1 checkpoint blocker was purchased from InVivoPlus (B7-H1, Bio cell, USA).
mouse anti-STAT3 (Invitrogen, cat number MA1-13042), rabbit anti-NF-κB (Life technologies, cat number 510500), mouse anti-PD-L1/CD274 (Proteintech, cat number 66248-1-Ig), and mouse anti-TGF (Invitrogen, cat number MA5-15065) were used for immunoblotting, and so were secondary antibodies goat anti-mouse and goat anti-rabbit Alexa® Fluor 555 (Thermo Fisher Scientific, Waltham). For immunoblotting, rabbit anti-actin (LI-COR Biosciences, Lincoln, Nebraska, USA) was used. The anti-mouse PD-L1 checkpoint blocker was purchased from InVivoPlus (B7-H1, Bio cell, USA).
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