LCMV RNA Generation and Reference

LCMV RNA reference was generated using a MEGAscript T7 kit (catalog number AM1333, Thermo Fisher Scientific) from LCMV NP gene DNA (position 1,713–2,238 in Genbank accession number JF912085) that was synthesized by Integrated DNA Technologies. The synthesized DNA with the T7 promoter sequence at the 5′ end was added. A plasmid containing part of the β-actin mRNA sequence (position 9–1,137 in Genbank accession number NM_001330273) was utilized as a reference for β-actin mRNA and genomic DNA.

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Misu M., Yoshikawa T., Sugimoto S., Takamatsu Y., Kurosu T., Ouji Y., Yoshikawa M., Shimojima M., Ebihara H, & Saijo M. (2023). Rapid whole genome sequencing methods for RNA viruses. Frontiers in Microbiology, 14, 1137086.

Publication 2023

MEGAscript T7 kit

Actin Gene Genomic Lcmv Mrna Plasmid

Corresponding Organization : National Institute of Infectious Diseases

Other organizations : Nara Medical University

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Variable analysis

independent variables

Synthesized DNA with the T7 promoter sequence at the 5′ end

dependent variables

LCMV RNA reference
β-actin mRNA and genomic DNA

control variables

MEGAscript T7 kit (catalog number AM1333, Thermo Fisher Scientific)
LCMV NP gene DNA (position 1,713–2,238 in Genbank accession number JF912085)
Part of the β-actin mRNA sequence (position 9–1,137 in Genbank accession number NM_001330273)

controls

Positive control: Plasmid containing part of the β-actin mRNA sequence
Negative control: Not explicitly mentioned

Annotations

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