Human scrambled shRNA, FIP200 shRNA, and Tsc1 shRNA encoded in pGIPZ lentiviral vector were purchased from Open Biosystems (Lafayette, CO). Mouse scrambled shRNA, LAL shRNA #1 and #2 were purchased from Sigma. Package vectors of pSPAX2 and pMD2.G were mixed with shRNA vectors and transfected in HK293 cells for virus production essentially as described before37 (link).
Protocol detail
Lentiviral shRNA Knockdown Protocol
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Corresponding Organization : University of Cincinnati Medical Center
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Variable analysis
independent variables
- Human scrambled shRNA
- FIP200 shRNA
- Tsc1 shRNA
- Mouse scrambled shRNA
- LAL shRNA #1
- LAL shRNA #2
- Not explicitly mentioned
- PSPAX2 (package vector)
- PMD2.G (package vector)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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