For in vivo CoQ repletion, littermates were randomly injected (ip) on P9 with 160 mg/kg CoQ10 (in intralipid) or equal volume vehicle (intralipid alone). CoQ10 (Sigma-Aldrich C9538) was dissolved in 100% ethanol and heated at 65°C for 5 minutes. Dissolved CoQ10 was then added to intralipid (Sigma-Aldrich I141) (2.9 mM) and heated for 5 minutes at 65°C (14 (link)). For dose-response testing, mouse pups were injected (ip) on P9 with 20, 40, 80, or 160 mg/kg CoQ10 (in intralipid) and assessed on P10. Equal volume vehicle (intralipid alone) served as a control injectate.
Protocol detail
In vivo CoQ10 repletion in mice
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Corresponding Organization : Columbia University Irving Medical Center
Other organizations : Yale University
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Variable analysis
independent variables
- CoQ10 dose (20, 40, 80, or 160 mg/kg)
- Unspecified
- Injection volume (equal to CoQ10 injection)
- Intralipid vehicle (no CoQ10)
- Positive control: CoQ10 injection
- Negative control: Intralipid vehicle injection
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