Quantitative PCR and Western Blot Analysis

qRT-PCR was performed using the TaqMan Gene Expression Master Mix (Thermo Fisher Scientific, #4369016), along with TaqMan Assays for TNFSF10 (Thermo Fisher Scientific, Hs00921974_m1), DDX58 (IDT, Hs.PT.58.4273674), IFIH1 (IDT, Hs.PT.58.1224165), OAS1 (IDT, Hs.PT.58.2338899), TNF (IDT, Hs.PT.5845380900) or CXCL8 (IDT, Hs.PT.5839926886). The fold change in expression of each gene was calculated using the ΔΔCT method, with RNA18S (IDT, Hs.PT.39a.22214856.g) as an internal control. To detect protein expression of TNFSF10, we conducted western blot analyses as previously described (36 (link)), using anti-TNFSF10 (Cell Signaling, #3219, 1:300 dilution), anti-tubulin (Cell Signaling, #2128, 1:2000 dilution) and anti-β-actin (abcam, #ab8227, 1:10 000 dilution) antibodies. After incubating with a fluorescent secondary antibody (Li-Cor, #926–68 071, 1:20 000 dilution), the fluorescence signals were detected by Li-Cor Odyssey Classic Infrared Imaging System, according to the manufacturer’s protocol.