Assessing Blood Microbial Diversity

Three ml of blood was collected from seven clinically healthy adults in Vacutainer tubes with heparin and K₃EDTA as anticoagulants (Vacutainer K3E and Vacutainer Heparin Tube, BD, USA). All samples were previously characterized for their microbial biodiversity content by applying 16S rDNA and ITS targeted next-generation sequencing (Panaiotov et al., 2021 (link)). Two hundred µl of all blood samples were tested for sterility by growing on Sabouraud and blood agar 90 mm plates at 37°C for 72 hours.

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Tsafarova B., Hodzhev Y., Yordanov G., Tolchkov V., Kalfin R, & Panaiotov S. (2023). Morphology of blood microbiota in healthy individuals assessed by light and electron microscopy. Frontiers in Cellular and Infection Microbiology, 12, 1091341.

Publication 2022

Vacutainer K3E

Adults Agar Anticoagulants Blood Heparin Rdna Sterility

Corresponding Organization : National Center of Infectious and Parasitic Diseases

Other organizations : Sofia University "St. Kliment Ohridski", Bulgarian Academy of Sciences, Institute of Neurobiology, South-West University "Neofit Rilski"

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Variable analysis

independent variables

Collection of blood samples from seven clinically healthy adults

dependent variables

Microbial biodiversity content of the blood samples as characterized by 16S rDNA and ITS targeted next-generation sequencing
Sterility of the blood samples tested by growing on Sabouraud and blood agar plates

control variables

Volume of blood collected (3 ml)
Anticoagulants used (heparin and K3EDTA)
Incubation temperature (37°C)
Incubation duration (72 hours)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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