The care and handling of the Xenopus model were approved by the National Research and Development Agency (ANID) and by the ethics, Care and Use of Animals committee of the University of Concepción (Fondecyt 11160562). The animals were treated following the ethical protocols established by the United States National Institute of Health (NIH) and the amphibian euthanasia protocol was based on that described by [104 (link)].
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