Viability Assessment of M. tuberculosis in Urine

Solid culture in Middlebrook 7H11 was used to assess the viability of M. tb spiked in urine samples. A total of 0.1 mL was inoculated into each compartment of the Middlebrook 7H11. The media was supplemented with OADC, 0.5% (v/v) of glycerol, and a cocktail of antibiotics consisting of Polymyxin, Amphotericin, Carbenicillin, and Trimethoprim (PACT). The plates were incubated at 37 °C for a maximum of 6 weeks. Growth of M. tb colonies was observed weekly, and colony count was conducted from a dilution with 10–200 colonies.

Free full text: Click here

Mapamba D.A., Sauli E., Lalashowi J., Buza J., John J., Mwaisango Z., Tarmo P., Sabi I., Rachow A., Ntinginya N.E, & Mtafya B. (2023). Performance of Tuberculosis Molecular Bacterial Load Assay Compared to Alere TB-LAM in Urine of Pulmonary Tuberculosis Patients with HIV Co-Infections. International Journal of Molecular Sciences, 24(4), 3715.

Publication 2023

Amphotericin Antibiotics Carbenicillin Dilution Glycerol Polymyxin Trimethoprim Urine

Corresponding Organization : Nelson Mandela African Institution of Science and Technology

Other organizations : Ludwig-Maximilians-Universität München, German Center for Infection Research

Top 5 similar protocols

Variable analysis

independent variables

Inoculation of Middlebrook 7H11 media with 0.1 mL of urine samples spiked with M. tb

dependent variables

Growth of M. tb colonies observed weekly
Colony count conducted from a dilution with 10–200 colonies

control variables

Middlebrook 7H11 media supplemented with OADC, 0.5% (v/v) of glycerol, and a cocktail of antibiotics (PACT)
Incubation of plates at 37 °C for a maximum of 6 weeks

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!