The industrially extracted mimosa (Acacia mearnsii) tannin powder used in the current study was obtained from UCL Tannin Company Pty (Ltd), Kwa-Zulu Natal, South Africa. The extract was obtained from the bark of a Acacia mearnsii tree through a series of hot water extraction processes at a controlled temperature, pressure, and time, followed by vacuum evaporation and subsequent air-drying into fine powder before packaging and storage at −4 °C [16 (link)]. The molecular weight of the mimosa ranged from 500–3000 Daltons [24 (link)]. The organic solvents used were: ethyl acetate and pentanol, procured from Sigma Aldrich, Johannesburg, South Africa. According to Missio et al. [16 (link)], pentanol has a polarity index of 0.568, whereas ethyl acetate has a polarity index of 0.228. Tannic acid, Folin–Ciocalteu reagent, butanol, and all other reagents used were of analytical grades supplied from Sigma Aldrich, Johannesburg, South Africa.
Free full text: Click here
