HPTLC Analysis of Prunus domestica Extracts

For HPTLC analysis, CAMAG Linomat V (CAMAG) applicator with precoated silica gel F₂₅₄ TLC-plates (Merck, Darmstadt, Germany) was used. The extracts of P. domestica fruit in different solvents (chloroform, methanol, water:alcohol (1:1; v/v) and water) were concentrated using a rotary evaporator and dried under reduced pressure using N₂ gas inert condition. Finally, 5 mg/mL stock solution of each extract was prepared and spotted on TLC plates. For analysis, properly diluted stock solution was spotted via TLC plates, followed by the development of TLC plates in toluene:ethyl acetate:formic acid (5:4:0.5; v/v/v) solvent system for chloroform, methanol, aqueous:alcohol (1:1; v/v) extracts, whereas butanol:acetic acid:water (8:2:2; v/v/v) solvent system was used for aqueous extract TLC plate development. TLC plates were scanned by CAMAG scanner-3 at 366 nm, and photographs of the chromatograms were saved [15 (link)].