Intrathecal Injection Protocol for Anesthetized Rats

After rats were anesthetized (2% isoflurane), a small slit was cut in the atlanto-occipital membrane, and a 6–7-cm piece of saline-filled PE-10 tubing was inserted. We confirmed intrathecal drug delivery by injecting lidocaine (400 µg/20 µl, Hospira, Lake Forest, IL), which resulted in a temporary motor paralysis of the lower limbs.^{19 (link),20 (link)}

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Tiwari V., Yang F., He S.Q., Shechter R., Zhang C., Shu B., Zhang T., Tiwari V., Wang Y., Dong X., Guan Y, & Raja S.N. (2016). Activation of Peripheral Mu-Opioid Receptors by Dermorphin [D-Arg2, Lys4] (1-4) amide Leads to Modality-preferred Inhibition of Neuropathic Pain. Anesthesiology, 124(3), 706-720.

Publication 2015

lidocaine

Drug delivery Forest Isoflurane Lidocaine Lower limbs paralysis Membrane Rats Saline

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Variable analysis

independent variables

Intrathecal drug delivery of lidocaine (400 µg/20 µl)

dependent variables

Temporary motor paralysis of the lower limbs

control variables

Anesthesia with 2% isoflurane
Cutting a small slit in the atlanto-occipital membrane
Inserting a 6–7-cm piece of saline-filled PE-10 tubing

controls

Positive control: Intrathecal injection of lidocaine (400 µg/20 µl), which resulted in temporary motor paralysis of the lower limbs.
Negative control: Not explicitly mentioned.

Annotations

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