Quantifying Cell Invasion and Colony Formation

Colony formation assays in soft agar were performed in triplicate as described earlier [43 (link)]. Images were captured by Olympus IX81 microscope using Image-Pro Plus software (Olympus) at 100× optical magnification and colony-forming efficiency was quantified. Matrigel invasion assay was performed as described previously [44 (link)]. Images were captured by Olympus IX81 microscope at 40X magnification. Statistical analysis was performed by Student’s t test using GraphPad software with level of significance P <0.001 (GraphPad Software, San Diego, CA, USA).

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Guturi K.K., Sarkar M., Bhowmik A., Das N, & Ghosh M.K. (2014). DEAD-box protein p68 is regulated by β-catenin/transcription factor 4 to maintain a positive feedback loop in control of breast cancer progression. Breast Cancer Research : BCR, 16, 496.

Publication 2014

IX81 microscope Image-Pro Plus software

Agar Assay Matrigel Microscope Optical Student

Corresponding Organization :

Other organizations : Indian Institute of Chemical Biology, Council of Scientific and Industrial Research

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Variable analysis

independent variables

Colony formation assays in soft agar
Matrigel invasion assay

dependent variables

Colony-forming efficiency
Invasion through Matrigel

control variables

Triplicate experiments for colony formation assay
Olympus IX81 microscope used for image capture
Image-Pro Plus software used for image analysis
Student's t-test used for statistical analysis
Significance level set at P < 0.001

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