Immunohistochemistry was performed as described previously 43 (link). Bones were decalcified in 10% EDTA for 2 weeks before processing. Tumors or prostate tissues were fixed in 4% PFA, transferred to 70% ethanol, and trimmed before standard histological processing, sectioning, and H&E staining performed by Histoserv Inc. (Germantown, MD). H&E and IHC slides were scanned using a Carl Zeiss AxioScan Z1 microscope with a 20X/NA 0.8 plan apochromat objective (Zeiss, Germany). The scale bars in the figures indicated the power of magnification at which the images were captured from the virtual microscope slides.
Protocol detail
Histological Analysis of Bone and Tumor Samples
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : National Cancer Institute, Nanjing University, Zhejiang Provincial Hospital of TCM, University of California, Los Angeles
Top 5 similar protocols
Variable analysis
independent variables
- Decalcification in 10% EDTA for 2 weeks before processing
- Immunohistochemistry (IHC) staining
- H&E staining
- Tissue fixation in 4% PFA
- Transfer to 70% ethanol
- Trimming before standard histological processing, sectioning, and staining
- No positive or negative controls were explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!